The herpes simplex is a acute herpetic skin disease that caused by herpes simplex virus. The herpes simplex virus has two types, the herpes simplex virus type I (HSV I) and the herpes simplex virus type II (HSV II). HSV I infects skin, mucous membrane (oral cavity mucous membrane), and organs (brain) other than the reproductive organs. HSV II infects skin and mucous membrane of the reproductive organs. The HSV infects the human body through respiratory tract, oral cavity, mucous membrane of the reproductive organs, and lives in the normal human mucous membrane, blood, saliva, and sensory ganglion. When the body’s resistance reduces, like fever, functional gastrointestinal disorder, menstruation, pregnancy, focal infection and mood changing，the HSV becomes activate. This kit is used for detect HSV I-IgG antibodies in the serum or plasma and to assist the epidemiological investigations of HSV I infected individuals.
1 Temperature Balancing: Take the assay kit out from the refrigerator and wait until it reaches to the room temperature.
2 Adding Diluent: Dilute the concentrated washing solution 20 times with purified or deionized water and shake well.
3 Adding Sample: Leave an empty well without adding any liquid (including any enzyme). Also leave 3 wells for negative control and 2 wells for positive control and 2 wells for cutoff control. Add 100μl each of above mentioned controls to their corresponding wells. Add 100μl of sample diluent to the rest of the wells prior to orderly adding 10μl of testing sample to each well and mix well.
4 Incubation: Cover the microplate with cover membrane and incubate for 30 minutes in oven or water bath set for 37℃±1℃.
5 Washing: Discard all the solution from the microplate fill up each will with washing solution. Wait for 30 seconds and discard all the solution from the microplate. Repeat the washing step 5 times and pat dry the microplate with a paper towel. Alternatively, use a micro plate washer to wash the plate 5 times and pat dry the plate with a paper towel.
6 Add Enzyme: Add 100ml enzyme complexes to each well (apart from the controls) and mix well by oscillating.
7 Incubation: Cover the microplate with the cover membrane and incubate at 37℃±1℃ for 30 minutes.
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